Retrouvez ici le mini livre des résumés des 1ières JS Numériques du RFMF
Conférence pleinière / Pleanry Talk
Vendredi 26 juin 2020 14h30
https://global.gotomeeting.com/join/478361621
Dr. RuAngelie Edrada-Ebel
Using Concatenated Analytical Data for Metabolomic Guided Production and Targeted Isolation of Bioactive Natural Products
High resolution Fourier transform mass spectrometric (HRFTMS) and nuclear magnetic resonance (NMR) spectroscopic data were employed as complimentary metabolomic tools to dereplicate chemical profiles of Bioactive Natural Products from their sources. The innovative strategy enhanced targeted cultivation, harvest, and isolation of biologically active compounds. Principal Component (PCA), Hierarchical Clustering (HCA), and Orthogonal Partial Least Square-Discriminant (OPLS-DA) analyses were used to evaluate HRFTMS and NMR spectral data of culture extracts. The results of the statistical analysis identified and validated the best culture conditions and extraction procedure which optimized the isolation of novel bioactive metabolites.
Novel secondary metabolites were screened using high resolution mass spectrometry and NMR-based metabolomics approaches. Metabolomic profiling using 2D-NMR and HR-ESIFTMS were done at different stages of the growth phase for both solid and liquid culture media. Multivariate analysis of fused data sets resulted to easier-to-interpret linear trends. The optimised method in terms of media, incubation time, and maximum production bioactive compounds are taken into account for the scale-up. Metabolomic- and bioassay-guided isolation was carried out to target the compound(s) of interest. With the metabolomics approach, it was possible to predict and optimise the production of the target secondary metabolite."
